Home - Sitemap - Contact Us




Confocal\Multiphoton
Spinning Disk Confocal

AxioObserver
AxioImager
Olympus Upright
Stereomicroscope

Olympus IX81
In Vivo
Brightfield
Live Specimen Imaging
Whole Slide Scanning
Specialty Techniques
Tissue Culture
Image Analysis
Courses
Policies
Applications
Links
Contact Us
Booking Calendar

How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


Nikon TIRF System

TIRF is a technique used to image a thin region (less than 200nm) close to the surface of a specimen at high resolution. This is useful in cell and molecular biology to observe events occurring on cellular surfaces such as cell adhesion, binding of hormones, secretion of neurotransmitters, and membrane dynamics.

On a TIRF microscope, the illumination and excitation light is directed at such an angle to the sample that it is totally internally reflected. When this happens, there is a small evanescent wave that passes through the sample and allows for the selective excitation of the fluorophores in a restricted region of the specimen immediately adjacent to the glass-water interface. The associated electromagnetic field decays exponentially from the interface, and thus penetrates to a depth of only approximately 100 nm into the sample.


Training Courses Available
--------------------------------------------------------------------------------
Widefield Microscope Training
Advanced Widefield Techniques

Model
--------------------------------------------------------------------------------
Nikon TE2000

Objective Lenses
--------------------------------------------------------------------------------
Plan Fluor 10x /0.25 Ph1
Plan Fluor 40x /0.6 Ph2
Plan Apo 20x/0.75 Ph2
Apo TIRF 60x /1.49 oil

Illumination Sources
--------------------------------------------------------------------------------
Xenon
488nm Argon laser

Filter Sets
--------------------------------------------------------------------------------
Chroma DAPI/FITC/Cy3/Cy5 Quad Sedat (only available for standard widefield mode) GFP (TIRF mode only)

Cameras
--------------------------------------------------------------------------------
Hamamatsu ORCA-ER

Software
--------------------------------------------------------------------------------
Metamorph Advanced

Advantages and Techniques
--------------------------------------------------------------------------------
Perfect Focus System
Prior motorized stage II
Environmental Chamber



Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.