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O-MUG

Comprehensive Course on Fluorescence Microscopy


WaveFX Spinning Disk Confocal

The Yokogawa spining disk confocal microscope provides a tradeoff between confocality, light output and speed. It's fast acquistion speeds makes is ideal for live cell applications. The unit contains a disk with many small pinholes and a disc above it that contains microlenses that focus the light on to these pinholes below.



Model
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Leica DMI 6000B (inverted) microscope

Illumination Sources
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405, 491, 561, 644nm
X-Cite 120

Objectives
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HCX PL FLUOTAR 10x/0.30
HCX PL FLUOTAR-L 20x/0.40 CORR
HC PLAN APO 20x/0.70
HCX PL FLUOTAR-L 63x/0.70 CORR (0.1-1.3)
HCX PL APO 40x/1.25-0.75 oil
HCX PL APO 63x/1.40-.6 oil CS

Camera
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Hamamatsu C9100-13 EMCCD (ImagEm)

Software
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Volocity

Additional components
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Chamlide stage-top incubator


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