Home - Sitemap - Contact Us


Equipment

Fundamentals of Microscopy
Basic Widefield Microscopy
Advanced Widefield Microscopy Techniques
Spinning Disk Confocal Microscopy
Confocal/Multiphoton Microscopy
Advanced Confocal/Multiphoton Microscopy Techniques
InVivo Imaging Training

Live-Specimen Imaging
Laser Capture Microdissection
Laser Scanning Cytometry
Image Analysis
Comprehensive Course on Fluorescence Microscopy
International Microscopy Visitors Program
Policies
Applications
Links
Contact Us
Booking Calendar

How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


Advanced In Vivo Imaging Techniques

Approximate Time:
Pre-requisite: In Vivo Imaging Training
__________________________________________________________________________

This course is an extension of the basic in vivo imaging training course. It covers more advanced imaging techniques on the following systems:

  • IVIS Spectrum:
    • Acquiring Time Sequences - using the imaging wizard
    • Translumination - this involves illuminating the specimen from below and allows for more sensitive detection and accurate quantifi­cation of deep sources
    • Spectral Un-mixing - this is good for separating signals of interest from autofluorescence or other overlapping signals, allowing for better detection of multiple reporters
    • Bioluminescence tomography (DLIT) or fluorescence tomography (FLIT) - allows for 3D localization and quantification
  • VisEn:
    • 3D fluorescence tomography - for 3D localization and quantification
  • Maestro:
    • Spectral Un-mixing - allows for separation of multiple probes and autofluorescence

Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.