Malignant prostate cancer cells fixed and stained immunofluorescently for phosphoserine 1981-ATM (red) and phosphothreonine 68-Chk2 (green). These two nuclei are undergoing mitosis, with mitotic chromosomes lines up at the metaphase plate (blue). Both ATM and Chk2 are found at the centromeres, of which there are 2, and in this picture, Chk2 and ATM are interacting at the centromere, resulting in yellow focal staining. 3D rendering of Z stacked images from DSU.
This image was taken on the AOMF Spinning disk confocal microscope.

-- Submitted by Farid Jalali, Bristow lab, OCI, PMH.