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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


AxioObserver

This inverted motorized microscope is ideal for live-cell imaging applications. Multi-channel, time lapse, and Z-stacks can be simultaneously programmed using Metamorph software. The AxioObserver has an excellent selection of high-quality objective lenses that can be used for both transmitted and fluorescence images. Motorized filters and shutters enable rapid switching between fluorophores during image acquisition. The Andor Zyla camera provides high resolution (5 megapixel), fast frame rates (up to 100 frames per second) and great sensitivity. An objective heater and stage top incubator with CO2 for live cell imaging is available upon request.

Training Courses Available
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Widefield Microscope Training
Advanced Widefield Microscope Techniques
Live Cell Imaging

Model
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Zeiss AxioObserver

Objective Lenses
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5x/0.25 Fluar
10x/0.30 EC Plan-Neofluar
20x/0.8 Plan-Apochromat
32x/0.4 Ph1 LD A-Plan
40x/0.95 Pol Plan-Apochromat with coverslip correction collar (0.13-0.21)
63x/1.4 Oil DIC Plan-Apochromat

Illumination Sources
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EXFO Series 120Q Fluorescence Illumination Unit
100W Halogen Lamp

Filter Sets
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DAPI 375nm excitation filterset
CFP 436nm excitation filterset
EGFP/FITC/Cy2 470nm excitation filterset
YGFP 500nm excitation filterset
Cy3/TRITC 545nm excitation filterset
TexasRed/mCherry 560nm excitation filterset
Cy5 620nm excitation filterset

Cameras
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Hamamatsu OCRA Flash4

Software
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Zeiss ZEN

Advantages and Techniques
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High sensitivity fluorescence imaging
Automated live cell imaging
Stagetop heater plus CO2


Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.