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O-MUG

Comprehensive Course on Fluorescence Microscopy


Axiovert

This fully automated inverted deconvolution microscope is ideal for demanding live-cell imaging applications. Multi-channel, time lapse, Z-stack and stage positioning can be simultaneously programmed using Image Pro-Plus. The Axiovert has an excellent selection of high-quality objective lenses that can be used for both transmitted and fluorescence images. Motorized filter blocks and shutters enable rapid switching between fluorophores. The Roper Scientific CoolSnap HQ CCD camera is sensitive enough to capture weak fluorescence signals, while the Coolsnap CF colour CCD camera is meant for transmission imaging of stained specimens (i.e., H&E). Objective and stage heaters are available upon request.


Training Courses Available
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Axiovert Training I: Introduction to the Axiovert 200M
Axiovert Training II: Advanced Fluorescence Acquisition

Model
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Zeiss Axiovert 200M

Objective Lenses
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FLUAR 5x/0.25 NA
FLUAR 10x/0.50 NA
FLUAR 20x/0.75 NA(DIC)
A-Plan 32x/0.40 NA Ph I
Plan-NEOFLUAR 40x/0.85 NA Pol(DIC)
NeoFluor 63x/1.25 NA oil immersion(DIC)
Plan-Apo 63x/1.4 NA oil immersion(DIC)

Illumination Sources
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EXFO X-Cite 120 Fluorescence Illumination System
HAL 100 Halogen

Filter Sets
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DAPI 360nm excitation filterset
PPIX 405nm excitation filterset
CFP 436nm excitation filterset
FITC 480nm excitation filterset
YGFP 500nm excitation filterset
Cy3 545nm excitation filterset
Cy5 640nm excitation filterset

Cameras
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Roper Scientific Coolsnap HQ (high sensitivity monochrome for fluorescence imaging)
Roper Scientific Coolsnap CF

Software
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Media Cybernetics Image Pro PLUS

Advantages and Techniques
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High sensitivity fluorescence imaging
Automated live cell imaging
Stagetop heater plus CO2
Deconvolution


Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.