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Confocal/Multiphoton

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O-MUG

Comprehensive Course on Fluorescence Microscopy


Zeiss LSM700 Confocal

Confocal fluorescence microscopes can generate high-resolution three-dimensional images of thick specimens. Our Zeiss LSM700 is equipped with solid state lasers, providing excitation lines from UV to red (405nm, 488nm, 555nm, 639nm). The instrument has an inverted microscope base, allowing imaging of live cells in coverglass-bottom petri dishes or chambers. It has two highly sensitive PMT detectors as well as spectral detection with a resolution of down to 1nm. Using the 63x/1.2NA water-immersion objective lens, the maximum resolution is about 0.2 microns laterally and about 0.5 microns in depth. Our Two-Photon system is also a fully loaded confocal microscope.

Training Courses Available
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Confocal/Multi-photon Microscope Training
Advanced Confocal/Multi-photon Imaging Techniques

Model
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Zeiss LSM700 Confocal Microscope

Objective Lenses
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FLUAR 5x/0.25 NA
FLUAR 10x/0.50 NA
Plan-Apochromat 20x/0.8 NA
Plan-Apochromat 40x/1.4 NA oil immersion
Plan-Apochromat 63x/1.4 NA oil immersion
C-APO 63x/1.2 NA water immersion

Illumination Sources
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Four solid state laser lines: 405nm, 488nm, 555nm, 639nm
X-Cite fluorescence light source for visual mode

Detection
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2 Channel reflected/fluorescence mode detection, transmitted light PMT, spectral detection

Acquisition Software
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LSM Zen 2012

Advantages and Techniques
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High resolution fluorescence imaging
Optical sectioning of thick fluorescent specimens


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