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Advanced InVivo Imaging Techniques
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Comprehensive Course on Fluorescence Microscopy
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O-MUG

Comprehensive Course on Fluorescence Microscopy


Fundamentals of Microscopy

Approximate Time: 2 hours
Pre-requisite: None
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There is much more to microscopy than meets the eye. Knowing how to properly operate and align a microscope is an invaluable skill that will ultimately make the difference between a poor and excellent image for both quantification and publication. There are also techniques such as differential interference contrast, darkfield and phase contrast that can drastically improve image contrast for certain specimens. Over the years, other components have been added to the standard upright microscope, further increase flexibility. Many microscopes today are equipped with mercury light sources and filters for fluorescence. Additionally, CCD cameras have all but replaced the standard 35mm cameras. Topics covered include:

  1. Microscope Components
  2. Transmission Light Path
  3. Koehler Illumination
  4. Resolution and Contrast
  5. Magnification and Numerical Aperture
  6. DIC, Phase Contrast, Darkfield
  7. Fluorescence Microscopy
    • Light Path
    • Filter Sets - excitation, dichroic, emission
    • Photobleaching
    • Optimizing Fluorescence Image
  8. CCD Cameras and Image Acquisition
  9. Various Microscope Types
  10. Microscope Care and Maintenance

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.


Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.