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Basic Widefield Microscopy
Advanced Widefield Microscopy Techniques
Spinning Disk Confocal Microscopy
Confocal/Multiphoton Microscopy
Advanced Confocal/Multiphoton Microscopy Techniques
InVivo Imaging
Advanced InVivo Imaging Techniques
Live-Specimen Imaging
Laser Capture Microdissection
Laser Scanning Cytometry
Image Analysis
Comprehensive Course on Fluorescence Microscopy
International Microscopy Visitors Program
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Comprehensive Course on Fluorescence Microscopy

Fundamentals of Microscopy

Approximate Time: 2 hours
Pre-requisite: None

There is much more to microscopy than meets the eye. Knowing how to properly operate and align a microscope is an invaluable skill that will ultimately make the difference between a poor and excellent image for both quantification and publication. There are also techniques such as differential interference contrast, darkfield and phase contrast that can drastically improve image contrast for certain specimens. Over the years, other components have been added to the standard upright microscope, further increase flexibility. Many microscopes today are equipped with mercury light sources and filters for fluorescence. Additionally, CCD cameras have all but replaced the standard 35mm cameras. Topics covered include:

  1. Microscope Components
  2. Transmission Light Path
  3. Koehler Illumination
  4. Resolution and Contrast
  5. Magnification and Numerical Aperture
  6. DIC, Phase Contrast, Darkfield
  7. Fluorescence Microscopy
    • Light Path
    • Filter Sets - excitation, dichroic, emission
    • Photobleaching
    • Optimizing Fluorescence Image
  8. CCD Cameras and Image Acquisition
  9. Various Microscope Types
  10. Microscope Care and Maintenance

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.

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