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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


iCys Laser Scanning Cytometer


The iCys Research Imaging Cytometer by Compucyte (http://www.compucyte.com) combines the precise quantification of laser-based cytometry, the high content of automated image analysis, and the visualization and sample manipulation benefits of inverted microscopy in one powerful instrument. These capabilities allow users to simultaneously assess multiple parameters and relate them back to their spatial locations. Applications of this technology include cell cycle/DNA content, viability, proliferation, toxicity, translocation, signalling, and more. This technology allows you to make flow cytometry measurements with the added power of microscopic visualization, all without trypsanizing your cells.

The system accommodates various samples including fixed and live cells, tissue sections and microarrays and provides analysis of both fluorescently and/or chromatically stained (brightfield) samples.

Example applications:

http://www.compucyte.com/applicationsoverview.htm

Training Courses Available
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Laser Scanning Cytometry

Model
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CompuCyte iCys Research Imaging Cytometer

Objective Lenses
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Illumination Sources
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Lasers:
  • Violet 405nm
  • Argon 488nm
  • HeNe 543nm
Epifluorescence source

Detectors
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4 PMTs plus a forward scatter detector

Advantages and Techniques
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Autofocus

Software
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iBrowser Software

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