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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


Nikon A1R Resonance Scanning Confocal

The Nikon A1R+ has two imaging modes. The resonance scanning mode uses an ultra-fast scanner that can image up to 420fps (with a 512x32 pixel size) and 30 fps (with 512 x 512 pixel size) making it ideal for imaging live cells and their dynamic processes. The galvano scanning mode allows for high resolution imaging of fixed and live cells and tissues.

This microscope also has a perfect focus system that allows focus to be maintained while doing long term live cell experiments.


Training Courses Available
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Nikon A1R microscope training
Live-cell imaging

Model
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Nikon A1R+ system is built onto a Nikon Eclipse TI-E inverted microscope

Software
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Nikon NIS Elements C for acquisition and analysis

Lasers
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405nm (20mW), 445nm (20mW), 488nm (20mW), 515nm (20mW), 561nm (20mW), 640nm (20mW)

Other Light Source
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X-Cite 120LED Prior Transmitted LED

Objective Lenses
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CFI Plan Apochromat Lambda 10x NA 0.45 WD 4.00mm, DIC
CFI Plan Apo VC 20x NA 0.75 WD 1mm, DIC
CFI Plan Fluor 40x Oil NA 1.30 WD 0.20mm Eco-glass, DIC
CFI Apo 60x Oil NA 1.40 WD 0.14mm Lambda , Nano-crystal, DIC
CFI Plan Apo IR 60x Water Immersion (WI) NA 1.27 WD 0.17mm nano-crystal coat with correction collar 0.15-0.19mm, DIC

Ocular/Visual Mode Filters
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DAPI/Hoechst/Alexa Fluor 488
ECFP/Cerulean
EGFP/FITC/CY2/Alexa Fluor 488
TRITC/Cy3/TagRFP/Alexa Fluor 546

Detectors
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2 GaAsP and 2 high sensitivity PMTs

Dichroic mirrors
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Has dichroic mirrors for 458nm, 514nm, 561nm and 640nm

Emission filters
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450/50nm, 525/50nm, 600/50+685/70nm, 485/35nm, 488/10nm, 538/33+605/50


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