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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


Olympus Upright

This upright microscope is optimized for fast fluorescence tiling of whole slides, with an automated stage and Metamporh software. It can also be used just as a simple fluorescence microscope for acquiring single images.

Training Courses Available
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Widefield Microscope Training
Advanced Widefield Microscope Techniques

Model
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Olympus BX50

Objective Lenses
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Olympus UPlanSApo 10x/0.40
Olympus UPlanSApo 20x/0.75
Olympus UPlanFl 40x/0.75

Illumination Sources
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EXFO fluorescence unit
HAL 100 Halogen

Filter Sets
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Semrock Quad Sedat DAPI/FITC/TRITC/Cy5
Q-dot 525, 565, and 585nm
Cy7

Cameras
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Photometrics CoolSNAP HQ2(monochrome for fluorescence imaging)

Software
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Molecular Devices Metamorph

Advantages and Techniques
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Automated fluorescence tiling

For brightfield whole slide scanning, see our Aperio Scanscope XT.


Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.