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O-MUG

Comprehensive Course on Fluorescence Microscopy


Spinning Disk Confocal Microscopy Training

Approximate Time: 2 hours
Pre-requisite: None
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The spinning disk confocal microscopy provides a tradeoff between confocality, light output and speed. It is excellent for live cell applications where acquistion speed is important. We manage several of these microscopes (Olympus DSU, Yokogawa, and the WaveFX). Each one works a bit differently, but give similar results. Check out the spinning disk category in equipment list for more information. The systems are equipped for automated multi-color imaging and z-stack acquisition and well as 3D analysis and deconvolution.

The course outline is as follows:

1. Introduction to the equipment and startup
2. Selecting the appropriate calibrations/settings
3. Operating the microscope in visual mode (transmitted light and fluorescence)
4. Acquisition (transmitted light, widefield, and confocal)
5. Multicolor acquisition
6. Creating a confocal Z-stack
7. Cleanup and shutdown

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.


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