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O-MUG

Comprehensive Course on Fluorescence Microscopy


Spinning Disk Training I

Approximate Time: 2 hours
Pre-requisite:None
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This hands-on training session will introduce the user to the basic operations of our Olympus Spinning Disk Confocal microscope. Users will be trained on the Olympus IX81, which is equipped for transmitted light, widefield fluorescence and confocal fluorescence imaging. Topics covered in this course include:

1. Introduction to the equipment and startup
2. Selecting the appropriate calibrations/settings
3. Operating the microscope in visual mode (transmitted light and fluorescence)
4. Acquisition (transmitted light, widefield, and confocal)
5. Multicolor acquisition
6. Creating a confocal Z-stack
7. Cleanup and shutdown

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.


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