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How to get started at the AOMF


Comprehensive Course on Fluorescence Microscopy

What's New

October 21, 2010 New Overnight booking policies
Overnight is defined as sessions that begin after 2pm and last for at least 12 hours. Overnight sessions receive a 50% discount on the entire session, but you must remember to choose the "Overnight" category when booking to receive this discount. If the microscope is left unattended overnight, users must return before the end of their booked timeslot to remove their sample, save their data, and clean the microscope area as needed. For example, if you want to book the Yokogawa Spinning Disk Confocal for a 12-hr multi-location timelapse experiment, you could book a slot from 16:00 to 9:00 with the "Overnight" category: the first hour (16:00-17:00) is to setup the microscope, then the 12-hr experiment runs to 5:00 in the morning; you come in at 9:00 to save the data and remove your sample, finishing by 9:00.

October 5, 2010
    Encryption at UHN: How will this affect the AOMF?
  • The AOMF computers will remain un-encrypted.
  • UHN users will no longer be able to map their network drives on the AOMF computers to transfer data.
  • The best way to transfer data from the AOMF computers is to bring an un-encrypted USB key or hard drive. Note that this device will be read only on an encrypted RIS computer.
  • Users are forbidden from creating, copying or storing Patient Health Information (PHI) on AOMF computers.
November 5, 2007
October 15, 2007
  • CICF - AOMF merger: We are excited to announce that the Centre for Imaging Cellular Function at TGH will be merging with the Advanced Optical Microscopy Facility (AOMF) at PMH to form the UHN-AOMF. The directors and managers of these facilities see substantial advantages in terms of resource and personnel utilization, cost effectiveness of operation and potential for future development if the two facilities are amalgamated under a single administrative structure. While there are still some formalities required before the merger is official, we’re proceeding with some administrative aspects of the merger over the next few weeks. Stay tuned!
  • Olympus will be demoing the Nanozoomer on Oct 16th at the AOMF. The Nanozoomer is capable of brightfield and fluorescence whole slide scanning.
August 3, 2007
  • Due to the ongoing problem with the confocal and two-photon microscopes being overbooked, we have decided to change the booking policies. We will be getting a new confocal microscope soon, but in the meantime, we hope that these policy changes will help people share time more on these instruments.The following are the booking policy changes that have been put into effect:
    1. You may now only book 7 hours of "Prime Time" per week on the Confocal and Two-Photon microscopes. On other instruments the 8-hour limit remains.
    2. "Prime Time" will now be redefined as 9am to 7pm, seven days per week for all instruments. The period from 7pm to 9am will be referred to as "After Hours".
    3. "After Hours" usage will receive a 25% discount on all instruments. To get the discount, you must make a separate entry in the booking calendar for the After Hours portion of your usage and choose the new "After Hours" category from the drop-down list.
May 25, 2007
  • Check out our O-MUG page for a summary of our next O-MUG meeting on June 4th, 2007.
May 16, 2007
  • We will be demoing the Nikon C1si confocal this week in our facility and there will also a presentation by Nikon this Friday May 11th in Rm 7-605 (PMH).

March 21, 2007
  • Recent changes to the online booking calendar:

    We have been having problems on the calendar with people messing up the am/pm options when booking the time slots. This prevents other people from booking the rest of the day. Also, as the microscopes are getting busier, people are starting to book time well in advance and then deleting their sessions at the last minute. This prevents other people from using the instruments. Finally, people often leave lamps/lasers on for the next person, which we encourage, but the problem is that some people don't end up showing up after hours so that the equipment stays on all night and even all weekend sometimes. So for people booking instruments after hours, the onus will be on you to ensure that the instruments are properly turned off, whether you show up or not. In light of these problems, we will be implementing the following changes to the booking calendar:
    • 24hour clock for booking time slots
    • You now cannot edit or delete a session with less than 24hrs notice
    • You now cannot book an instrument more than 3 weeks in advance
February 5, 2007
  • Olympus Canada will be giving a demo of Hamamatsu's new Nanozoomer Digital Patholgy system on February 12th and 13th. If you're interested in attending, please contact Chantal Blanchard from Olympus Canada.
  • We are still demoing the TISSUEscope II beta (Biomedical Photometrics Inc, BPI). This is a high throughput system for brightfield, fluorescence and confocal imaging of whole slides. If you're intersted, please contact Glenn Smith or Kavita Aswani to setup a demo. Also, for more info check out their website at www.confocal.com.
  • A summary of the last O-MUG meeting on FRET microscopy is now posted here.
November 20, 2006
Version 8 of Aperio ImageScope is now available! Some features of this version include:
  • Improvements to the Annotation window
  • Annotation link manager (allows you to link an annotation files to different image files)
  • Some new algorithms available
  • Access to images through server
  • Ability to run algorithms in batch mode on server
  • Option to save image views

Click here to download Ver8.

November 14, 2006
  • We've started planning our our first Optical Microscopy Users Group (O-MUG) meeting. Check out the O-MUG page for details!
  • We are currently demoing the TISSUEscope II beta (Biomedical Photometrics Inc, BPI) for a couple months. This is a high throughput system for brightfield, fluorescence and confocal imaging of whole slides. If you're intersted, please contact Glenn Smith or Kavita Aswani to setup a demo. Also, for more info check out their website at www.confocal.com.
November 1, 2006
  • A new image analysis station was added (Digital Darkroom II). This computer has more memory, making it faster for performing operations such as stitching together large images. Please contact us if you think you need access to this computer.
  • For the next two months we will be housing two new instruments: a cRI Maestro for in vivo fluorescence imaging and an Olympus spinning disk confocal for fast live cell imaging. Contact us for more details.
  • Check out our whole slide scanning instruments for brightfield (Aperio scanner) and fluorescence (Olympus Upright) imaging.
  • In the next few months, we will be starting up microscopy users group. Stay tuned for details!
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