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Comprehensive Course on Fluorescence Microscopy

Whole Slide Scanning/Tiling

With a regular microscope, you are often limited by the field of view of the objective as to how much of your tissue you can actually fit into an image. In our facility, we have several instruments that can be used for imaging a large region or entire section of tissue, through the process called whole slide scanning or tiling. Tiling basically involves setting up the areas of your slide that you would like to scan and then having the microscope take multiple images across your slide to cover that area. Then the images can be stitched together to get a larger image. In our facility, we have two instruments which are dedicated to tiling. The Aperio scanner, which we run as a full service instrument, can be used for scanning histology or pathology slides and our Olympus Upright microscope can be used for both histology slides and fluorescence slides.
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